Bile Salts Affect on Lipase Activity

s providele sore Salts And Lipase Activity The aim of this experiment is to invesitigate the erect of saddle sore salts on Lipase activity. Equiptment And Materials * establish Tube Rack with 3 Test Tubes * 3 5ml Syringes * 4 100ml Beakers * Dropping Bottle of cosmopolitan Indicator * Stopclock * optic Protection * Marker Pen * pee Bath set at a continuous Temperature of 37?C * 200ml Full Cream Milk * 100ml of 3% Bile Salt Solution * 50ml of 0.1M Sodium Carbonate Solution manner 1. Collect all materials indicated above. 2. using marker pen, label the tryout tubes with A, B and C. 3. correspond 5ml(5000µm) of draw to each turn out tube. 4. To test tubes A and C cast up 1ml(1000µm) of 3% rancor salts solution. To test tube B add 1ml(1000µm) of Distilled Water. 5. Add 1cm(1000µm) of global indicator to each test tube. 6. Add 0.1M sodium carbonate solution slowly drop by dro p until the confine of each tube are at pH7 (pale green in colour). 7. Add 2ml(2000µm) of the 5% Lipase solution to test tubes A and B and 2ml(2000µm) of distilled water to test tube C. 8. gamy shake the circumscribe of the tubes so that the contents are mixed.

moorage tubes into the water lavatory set at a constant temperature of 37?C. 9. befool a note of the colour of the universal indicator in each test tube at the start and afterward a period of 1 hr. geminate the colour with the pH colour chart to obtain pH values. Put results into a table and record class results also. Results. Test Tube| A| | B| | C| | Group| StartpH| Endp! H| StartpH| EndpH| StartpH| EndpH| 1| 7| 5.65| 7| 5.72| 7| 7.3| 2| 7| 6.65| 7| 5.65| 7| 6.65| 3| 7| 6.6| 7| 6.4| 7| 6.38| 4| 7| 5.3| 7| 5.26| 7| 7.17| 5| 7| 5.4| 7| 5.4| 7| 9.6| fair(a) pH| 7| 5.72| 7| 5.686| 7| 7.42| Bar represent of Results [ 1 ]If you want to get a full essay, influence it on our website: BestEssayCheap.com

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